Homepage of Limanskii Alex

CURRICULUM VITAE

Family name	Limanskii
First name	Alex
Date of birth	5 August, 1958
Place of birth	Kharkov, Ukraine
Citizenship	Ukraine
Marital status	Married, 1 daughter
Present address	Laboratory of Antimicrobal Drugs, Mechnikov Institute for Microbiology and Immunology, National Academy of Medical Sciences of Ukraine, 14 Pushkinskaya St., Kharkov, 61057, Ukraine
Home adress	47 Kosmicheskaya St., apt. 26, Kharkov, 61145, Ukraine; phone: (38) – 057 – 7022-243
Education	B.S.: 1975-1979, Radiophysics and Biophysics, Kharkov State University, Kharkov, (Ukraine). M.S.: 1979-1980, Molecular Biophysics, Kharkov State University, Kharkov, (Ukraine). Diploma title: Spectral and thermodynamic parameters of modified 6 - azauracils. Ph.D.: 1996-1998, Mechnikov Institute for Microbiology and Immunology, Kharkov, Ukraine. Thesis title: Detection of viruses and bacteria by polymerase chain reaction. Interaction of microorganisms nucleic acids with antimicrobial and anticancer drugs. Dr.Sci.: 2007, Palladin Institute of Biochemistry, National Academy of Sciences, Kiev, Ukraine. Thesis title: Modification and functionalization of probes and substrates for nanobiotechnology. Thesis (Russian) Thesis (English) Thesis (Ukrainian)
Academic appointments	2003 - 2013 - Chief Scientist, Mechnikov Institute for Microbiology and Immunology, National Academy of Medical Sciences of Ukraine, Kharkov, Ukraine. April 2004 – September 2004 - JSPS Postdoctoral Fellow , Graduate School of Biostudies, Kyoto University, Kyoto, Japan. 1996 - 2002 - Senior Scientist, Mechnikov Institute for Microbiology and Immunology, Ukrainian Academy of Medical Sciences, Kharkov. July 1998 - June 1999 - Postdoctoral Research Associate, Department of Microbiology, Arizona State University, Tempe, AZ, USA. 1996 - 2001 - Senior Scientist, Laboratory of Slow Infectious Diseases, Institute of Experimental and Clinical Veterinary Medicine, Ukrainian Academy of Agrarian Sciences. 1988 - 1996 - Researcher, Department of Biophysics, Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine. 1985 -1988 - Junior researcher, Department of Biophysics, Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences. 1980 - 1985 - Engineer, Department of Biophysics, Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences.
Other professional positions	1997 - 2000 - Chief Scientist, Institute of Cattle Breeding, Kharkov, Ukraine. 1995 - 1996 - Scientist, Regional Medical Genetic Center, Kharkov, Ukraine. 1993 - 1994 - Researcher, Regional Epidemiological Station, Kharkov, Ukraine.
Awards	1990 - 1992 - Research Grant, Ukrainian National Programme “Anti- HIV”: Study of HIV LTR by scanning tunneling microscopy. 1990 - 1991 - Research Grant, Ukrainian National Programme “Chernobyl” : PCR –detection of viral diseases. 1996 - 2011 - Research Grants of Ukrainian Ministry of Health and Ukrainian Academy of Medical Sciences upon study of genome organization of viral and bacterial pathogens.
Major research interests	nanotechnology and nanobiotechnology (atomic force microscopy (AFM) of cells, biopolymers, nucleic acids, chromatin; modification of AFM substrates and probes, adhesion and mechanical properties of cells, chemical force microscopy). molecular medicine (molecular typing of bacteria and viruses, design of molecular genetic test-systems for typing and detection of human and animal viruses and bacteria, sexing of animal embryos, preimplantation genetic diagnostics). molecular biology of nucleic acids, gene regulation, protein - DNA interaction (mechanisms of DNA - ligand specific interaction, thermodynamics of DNA and DNA - drug interaction). bioinformatics (genomics, phylogenetic analysis, search of molecular regulators in the bacterial and viral genomes).
Professional experience	Purification of nucleic acids; equilibrium centrifugation; HPLC and conventional liquid chromatography; spectrophotometry; thermodynamics of DNA melting; conventional gel electrophoresis; polymerase chain reaction; design of molecular genetic test-system for detection and differentiation of microorganisms; detection of human and animal viruses and bacteria; imaging cells and biomolecules in air and buffer solutions by atomic force microscopy; force measurements, modification of AFM probes and substrates in aminosilane vapours.
LIST OF MAIN PUBLICATIONS
1. Limanskii A., Sheina G., Stepanova T., Blagoi Yu. Studies of cationic and neutral forms of acridine compounds interaction with nucleic acids // Studia biophysica. – 1984. – V. 100, N 3. – P. 187-194.
2. Limanskaya O., Limanskii A., Bezugly N. Preimplantation embryo sexing of farm animals by poly-merase chain reaction. – Russian J. of Biotechnology 2000, 2: 66-72.
3. Limanskii A.P. Visualization of cruciform structure in supercoiled DNA by atomic force microsco-py. – Biophysics 2000, V. 45, N 6: 1039-1043.
4. Limanskii A., Limanskaya O. The methods of molecular genetics as the basis for the programs of bovine leukosis eradication. – Russian J. of Biotechnology 2001, 3: 187-193.
5. Limanskii A. Study of aminomodified mica as a substrate for nucleic acids atomic force microscopy. – Biopolymers and Cell 2001, V. 17, 4: 292 – 297.
6. Limanskii A. Study of aminomodified probes for atomic force microscopy of biomolecules. – Biopolymers and Cell 2002, V. 18, 1: 62-70.
7. Limanskii A., Limanskaya O. Comparison of primer sets for detection of bovine leukemia virus by polymerase chain reaction. – Bulletin of the Veterinary Institute in Pulawy (Poland) 2002, V. 46, 1: 27-36.
8. Limansky A., Shlyakhtenko L., Schaus S., Henderson E., Lyubchenko Y. Aminomodified probes for atomic force microscopy. – Probe Microscopy 2002, V. 2, 3-4: 227-234.
9. Lymans’kiy A.P., Lymans’ka O.Yu. Study of microorganisms genome DNA by atomic force microscopy. – Tsitologia i Genetika 2002, V. 36, N 4: 30-36.
10. A. Limanskii, L. Geue, O. Limanskaya, D. Beier Typing of bovine leukemia virus circulated in Ukraine. – Problems of Virology 2004, N 1: 39-44 (Russian).
11. Limanskii A., Minukhin V., Limanskaya O., Pavlenko N., Mishina M., Tsyganenko A. Species-specific detection of Proteus vulgaris and Proteus mirabilis by polymerase chain reaction. – J. of Microbiology, Epidemiology and Immunobiology 2005, N 3: 33-39.
12. Limanskii A. Oversupercoiling and compaction of supercoiled DNA. – Tsitologia i Genetika 2005, V. 39, N 2: 64-71.
13. Limanskaya O., Rola M., Bicka L., Kuzmak J., Limanskii A. Detection of bovine immunodeficien-cy virus proviral DNA by polymerase chain reaction. – Problems of Virology 2005, V. 50, N 2: 38-43.
14. Limanskii A. Visualization of amplicons after polymerase chain reaction. – Biophysics 2006, V.50, 6: 1019-1024.
15. Limanskaya L., Limanskii A. S-DNA is oversupercoiled with DNA 1.94 – 2.19 A rise per base pair along duplex axis. – Molecular Biology 2006, V. 40, N 1: 107-120.(https://commerce.metapress.com/content/n7p727u770m33185/resource-secured/?target=fulltext.pdf&sid=sz0cux55jyqorq332nhj2i55&sh=www.springerlink.com)
16. Limanskaya L,, Limanskii A. Compaction of single supercoiled DNA molecules adsorbed onto amino mica. – Russian Journal of Bioorganic Chemistry 2006, V. 32, N 5: 494-510.(https://commerce.metapress.com/content/4l37213537375625/resource-secured/?target=fulltext.pdf&sid=vaxansv1kcp41q45hdlpsf55&sh=www.springerlink.com)
17.Limanskii A. Functionalization of amino modified probes for atomic force microscopy. – Biophy-sics 2006, V. 51, 2: 186-195.
18. Limanskii A. Compaction of single molecules of supercoiled DNA: from duplex to minitoroidal and spheroidal conformations. – Biophysics 2007, V. 52, N 2: 252-260.
19. Limanskaya O., Limanskii A. Imaging of T7 RNA polymerase elongation complexes by atomic force microscopy. – Molecular Biology 2008, V. 42, N 3: 469-477. (http://dx.doi.org/10.1134/S0026893308030175)
20. Limanskaya O., Limanskii A. Imaging compaction of single supercoiled DNA molecules by atomic force microscopy // General Physiology and Biophysics. – 2008. – V. 27, N 4. – P. 322-337.
21. O.Yu. Limanskaya, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, X. Wu, J. Zhang, T.V. Fesenko, V.A. Pokrovskiy, A.P. Limanskii Identification of wild-type Mycobacterium tuberculosis isolates and point mutations associated with isoniazid resistance // Molecular Biology. – 2010. – Vol. 44, N 4. – P. 559–567.(http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1134/S0026893310040096)
22. O.Yu. Limanskaya, A.P. Limanskii. Distribution of potentially hairpin-loop structures in the genome of bovine retroviruses // Problems of Virology. – 2009. – V. 54, N 4. – P. 27-32.
23. O.Yu. Limanskaya, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, A.P. Limanskii Detection of wild type isolates and isoniazid-resistant mycobacteria tuberculosis // Tuberculosis and Lung Diseases. – 2010. – N 9. – P. 45-51.
24. O.Yu. Limanskaya, T.N. Fesenko, V.A. Pokrovskiy, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, A.P. Limanskii. Characterization of oligonucleotides with LNA monomers for PCR detection of point mutations in Mycobacterium tuberculosis genome // Biochemistry (Moscow). Supplement Series B: Biomedical Chemistry. – 2011. – V. 5, N 2. – P. 144–151.
25. Limanskaya O., Murtazaeva L., Limanskii A. Species-specific detection of causative agent of anthrax // Biotechnology. – 2012. – V. 5, N 1. – P. 92-99.
26. O. Limanskaya, A. Limanskii. Study of elongation complexes for T7 RNA polyme-rase // Biophysics. – 2012. – V. 57, N 4. – P. 428-441.
27. Limanskaya O., Murtazaeva L., Klee S., Limanskii A. Detection of anthrax causative agent by real-time polymerase chain reaction // Biotechnology. – 2012. – V. 5, N 5. – P. 65-71.
28. O. Limanskaya, L. Murtazaeva, A. Limanskii. Search for the new potential RNA thermometers in the genome of Salmonella enterica // Microbiology. – 2013. – V. 82, N1. – P. 69-78.
29. O.Yu. Limanskaya, L.A. Murtazaeva, and A.P. Limanskii. Potential thermosensitive riboswitches in the genome of Salmonella // Cytology and Genetics. – 2013. – V. 47, N 5. – P. 268–275.
PATENTS
1. Ukraine Patent 36260: A.P. Limanskii, O.Yu. Limanskaya Detection method of bovine leukemia virus 25.11.1999; Publ.16.04.2001. Bul. N 3.
2. Ukraine Patent 33324: A.P. Limanskii, O.Yu. Limanskaya, V.F. Babkin, N.D. Bezugly, Yu.L. Volyansky, I.Yu. Kuchma, A.I. Dobroskok, M.V. Babkin. Method of animal embryos sexing 10.02.1999. Publ.15.02.2001. Bul. N 1.
3. Ukraine Patent 40310: A.P. Limanskii, A. Yu. Volyansky, O.Yu. Limanskaya. Detection method of Listeria monocytogenes, L. Ivanovii, L.seeligeri, L.welshimeri 01.12.2000; Publ.16.07.2001.Bul. N 6.
4. Ukraine Patent 40371: A. Yu. Volyansky, A.P. Limanskii, O.Yu. Limanskaya. Detection method of pathogenic Listeria sp. 22.12.2000; Publ.16.07.2001. Bul. N 6.
5. Ukraine Patent 39715: O.Yu. Limanskaya, A. Yu. Volyansky, A.P. Limanskii. Typing method of pathogenic and nonpathogenic Listeria sp. 03.01.2001; Publ.15.06.2001. Bul. N 5.
6. Ukraine Patent 66255: Tsyganenko A., Limanskaya O., Limanskii A., Mishina M., Minukhin V. Detection method of Proteus vulgaris 09.09.2003; Publ.15.04. 2004. Bul. N 4.
7. Ukraine Patent 66291: Limanskii A., Limanskaya O., Volyanskii A., Dobroskok A., Novikov S., Manolov V., Volyanskaya N. Detection method of bovine immunodeficiency virus 13.10.2003; Publ.15.04. 2004. Bul. N 4.
8. Ukraine Patent 13571: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Limanskaya L., Klimov O. Method of mica (as substrate for atomic force microscopy) modification 22.08.2005; Publ.17.04.2006. Bul. N 4.
9. Ukraine Patent 17942: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Limanskaya L., Klimov O. Method of stretching linear and supercoiled DNA molecules 25.04.2006; Publ. 16.10.2006. Bul. N 10.
10. Ukraine Patent 17981: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Kuchma I., Limanskaya L., Pugach N. Method for compression of linear and supercoiled DNA molecules 03.05. 2006; Publ.16.10.2006. Bul. N 10.
11. Ukraine Patent 80264: Tsyganenko A., Limanskaya O., Limanskii A., Minukhin V. Detection method of Proteus vulgaris and Proteus mirabilis 10.06. 2004; Publ.10.09.2007. Bul. N 14.
12. Ukraine Patent 34435: Limanskii A., Limanskaya O, Limanskaya L. Differentiation method of mycobacteria tuberculosis complex 17.03. 2008; Publ.11.08.2008. Bul. N 15.
13. Ukraine Patent 70049: Limanskaya O., Murtazaeva L., Limanskii A. Detection method of anthrax causative agent 14.11.2011; Publ. 25.05.2012. Bul. N 10.

Return to Research group