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CURRICULUM VITAE
Family name   Limanskaya
First name   Olga
Date of birth   18 September, 1960
Place of birth   Kharkov, Ukraine
Citizenship   Ukraine
Marital status   Married, 2 daughters
Present address  National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, National Academy of Agrarian Sciences of Ukraine, 83 Pushkinskaya St., Kharkov, 61023, Ukraine
Home adress  47 Kosmicheskaya St., apt. 26, Kharkov, 61145, Ukraine; phone: (38) – 057 – 7022-243
Education
  • B.S.: 1977-1981, Radiophysics and Biophysics, Kharkov State University, Kharkov (Ukraine).
  • M.S.: 1981-1982, Molecular Biophysics, Kharkov State University, Kharkov (Ukraine).
  • Diploma title: Study of DNA-thiotepha interaction by temperature denaturation.
  • Ph.D.: 2001, Institute of Molecular Biology and Genetics, National Academy of Sciences, Kiev, Ukraine.
  • Thesis title: Design of molecular genetic test-systems for early detection of bovine leukemia virus and preimplantation embryo sexing.
  • Dr.Sci.: 2011, Palladin Institute of Biochemistry, National Academy of Sciences, Kiev, Ukraine.
  • Thesis title: Molecular technologies for typing and detection of bacterial and viral pathogens.
    • Academic appointments
  • 2002 - present - Chief Scientist, Laboratory of Molecular Diagnostics, National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, National Academy of Agrarian Sciences of Ukraine, Kharkov, Ukraine.
  • 2001 - present - Chief Scientist, Mechnikov Institute of Microbiology and Immunology, National Academy of Medical Sciences of Ukraine, Kharkov, Ukraine.
  • 2002 - 2003 - Senior Scientist, Department of Microbiology, Kharkov Medical University, Kharkov, Ukraine.
  • 1996 - 2002 - Scientist, Laboratory of Slow Infectious Diseases, Institute of Experimental and Clini- cal Veterinary Medicine, Ukrainian Academy of Agrarian Sciences, Kharkov, Ukraine.
  • 1988 - 1997 - Researcher, Department of Biophysics, B. Verkin Institute for Low Temperatures Phy- sics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.
  • 1986 -1988 – Junior researcher, Department of Biophysics, B. Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.
  • 1982 - 1986 - Engineer, Department of Biophysics, B. Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.
    • Other professional positions
  • 2002 - 2003 - Senior lecturer, Department of Biotechnology and Organic Chemistry, National Technical University “Kharkov Polytechnical Institute”, Kharkov, Ukraine.
  • 1999 - Scientist, Institute of Cattle Breeding, Kharkov, Ukraine.
  • 1995 - 1996 - Scientist, Regional Medical Genetic Center, Kharkov, Ukraine.
  • 1993 - 1994 - Researcher, Regional Epidemiological Station, Kharkov, Ukraine.
    • Awards
  • 1989 – A. Zubarev Prize of Kharkov Regional Young Scientists League.
  • 1990 - 1992 - Research Grant, Ukrainian National Programme “Anti- HIV”: Investigation of HIV LTR by scanning tunneling microscopy.
  • 1990 - 1991 - Research Grant, Ukrainian National Programme “Chernobyl”: PCR –detection of viral diseases.
  • 1993 – Individual J. Soros Grant.
  • 1996 - 2003 - Research Grants of Ukrainian Ministry of Health and Ukrainian Academy of Medical Sciences at study of genome organization of viral and bacterial infectious agents.
    • Major research interests
  • Bioinformatics – search of molecular markers for structural differentiation of viruses and bacteria genomes, molecular evolution and phylogenetic analysis.
  • Molecular retrovirology, molecular biology and molecular medicine – development of molecular technologies for detection and typing human and animal pathogens, gene regulation, sexing of animal embryos, preimplantation molecular diagnostics, protein - DNA interaction, mechanisms of DNA – drug specific interaction, thermodynamics of DNA and DNA – drug interaction.
  • Nanobiology – nanobiotechnological approaches for visualization and manipulation by single biomolecules and cells, atomic force microscopy of cells, biopolymers, nucleic acids, protein-nucleic acids complexes.
  • Mass spectrometry of biomolecules.
    • Professional experience  Purification of nucleic acids; equilibrium centrifugation; HPLC and conventional liquid chromatogra-phy; optical spectroscopy; thermodynamics of DNA melting; conventional gel electrophoresis; polymerase chain reaction; design of molecular genetic test-systems for detection and differentiation of microorganisms; detection of human and animal viruses and bacteria.
    LIST OF MAIN PUBLICATIONS
    1.Pyatigorskaya T., Zhilkova O., Arkhangelova N., Shelkovsky V., Sukhodub L. Study of thiotepha stability in aqueous and aqueous -salt solutions. - Chemical Pharmaceutical Journal. – 1984, N 3:343-349.
    2. Pyatigorskaya T., Zhilkova O., Shelkovsky V., Kosevich M., Sukhodub L. Study of thiotepha trans-formation products in aqueous solutions by thin layer chromatography and mass spectrometry. – Chemical Pharmaceutical Journal. – 1985, N 10: 1235-1241.
    3. Sukhodub L., Shelkovsky V., Kosevich M., Pyatigorskaya T., Zhilkova O. Mass spectroscopic stu-dy of interaction of thiotepha with nucleic acids. – Proceedings of Academy of Sciences of USSR. – 1985. – V. 283, N 3: 714-716.
    4. Pyatigorskaya T., Zhilkova O., Muravjeva L., Sukhodub L. DNA interaction with anticancer drug thiotepha. – Molecular Biology. – 1986. – V. 20, N 2: 423-429.
    5. Sukhodub L., Shelkovsky V., Kosevich M., Pyatigorskaya T., Zhilkova O. Nucleic acid base comp-lexes with thiotepa as revealed by field ionization mass spectrometry. – Biomedical and Environmen- tal Mass Spectrometry. – 1986. – V.13, N 4: 167-170.
    6. Pyatigorskaya T., Zhilkova O., Shelkovsky V., Arkhangelova N., Grizodub A., Sukhodub L. Hydro-lysis of 1,1’,1”-phosphinothioylidinetriaziridine (Thiotepha) in aqueous solution. – Biomedical and Environmental Mass Spectrometry. – 1987. – V. 14, N 4: 143-148.
    7. Sukhodub L., Andrievsky G., Pyatigorskaya T., Kosevich M., Zhilkova O. Application of mass spectrometry with atom ionization for product identification of DNA-thiotepha interaction. – Bioorganic Chemistry. – 1988. – V. 14, N 12:1698-1699.
    8. Sukhodub L., Kosevich M., Shelkovsky V., Pyatigorskaya T., Zhilkova O. Direct observation of nucleic acid bases adducts with thiotepha by ionization mass spectrometry. – Biophysics. – 1990. – V. 35, N 4: 549-552.
    9. Limanskaya O., Limanskii A. Universal test-system for PCR sexing of cattle, sheep, goat and pig embryos. – Journal Biology of Reproduction. – 1999. – V. 60, Suppl. 1. – P. 195.
    10. Limanskaya O., Limanskii A., Bezugly N. Preimplantation embryo sexing of farm animals by po-lymerase chain reaction. – Russian J. of Biotechnology. – 2000, N 2: 66-72.
    11. Limanskii A., Limanskaya O. The methods of molecular genetics as the basis for the programs of bovine leukosis eradication. – Russian J. of Biotechnology. – 2001, N 3: 187-193.
    12. Limanskaya O., Limanskii A. Analysis of primer sets for PCR-detection of provirus DNA of bo- vine leukemia virus and human immunodeficiency virus. – Biopolymers and Cell. – 2002. - V. 18, N 2: 124-130.
    13. Limanskii A., Limanskaya O. Comparison of primer sets for detection of bovine leukemia virus by polymerase chain reaction. – Bulletin of the Veterinary Institute in Pulawy (Poland). – 2002. – V. 46, N 1: 27-36.
    14. Lymans’kiy A.P., Lymans'ka O. Study of microorganisms genome DNA by atomic force micro-scopy. – Cytology and Genetics. – 2002. – V. 36, N 4: 30-36.
    15. Lymans'kyy A., Limans'ka O. Visualization of cyanobacteria in aqueous solution by atomic force microscopy. – Cytology and Genetics. – 2003. – V. 37, N 1: 68-71.
    16. Limanskii A., Geue L., Limanskaya O., Beier D. Typing of bovine leukemia virus circulated in Ukraine. – Problems of Virology. – 2004, N 1: 39-44.
    17.Limanskii A., Minukhin V., Limanskaya O., Pavlenko N., Mishina M., Tsyganenko A. Species-specific detection of Proteus vulgaris and Proteus mirabilis by polymerase chain reaction. – J. of Mic- robiology, Epidemiology and Immunobiology. – 2005, N 3: 33-39.
    18. Limanskaya O., Rola M., Bicka L., Kuzmak J., Limanskii A. Detection of bovine immunodeficiency virus proviral DNA by polymerase chain reaction. – Problems of Virology. – 2005. – V. 50, N 2: 38-43.
    19. Limanskaya O.Y., Limanskaya L.A., Limanskii A.P. Compaction of supercoiled DNA on modified aminomica. – Biopolymers and Cell. – 2006. – V. 22, N 1: 18-28.
    20. Limanskaya O., Limanskii A. Imaging of T7 RNA polymerase elongation complexes by atomic force microscopy. – Molecular Biology. – 2008 – V. 42, N 3: 469-477.
    21. Limanskaya O., Limanskii A. Imaging compaction of single supercoiled DNA molecules by atomic force microscopy. – General Physiology and Biophysics. – 2008. – V. 27, N 4: 322-337.
    22. Limanskaya O.Yu., Limanskii A.P. Distribution of potentially hairpin-loop structures in the genome of bovine retroviruses. – Problems of Virology. – 2009. – V. 54, N 4: 27-32.
    23. Limanskaya O.Yu. Species-specific detection of Mycobacterium tuberculosis complex. – Tuberculosis and Lung Diseases. – 2009, N 10: 49-55.
    24. Limanskaya O.Yu. Bioinformatic analysis of inverted repeats of coronaviruses genome. – Biopolymers and Cell. – 2009. – V. 25, N 4: 307-314.
    25. Limanskaya O.Yu. Polypurine/polypyrimidine sequences with the potential of forming triplexes in the proviral DNA of bovine retroviruses. – Cytology and Genetics. – 2010. – V. 44, N 1: 10-18.
    26. O.Yu. Limanskaya, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, X. Wu, J. Zhang, T.V. Fesenko, V.A. Pokrovskiy, A.P. Limanskii. Identification of wild-type Mycobacterium tuberculosis isolates and point mutations associated with isoniazid resistance. – Molecular Biology. – 2010. – Vol. 44, N 4: 559–567.
    27. O.Yu. Limanskaya, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, A.P. Limanskii Detection of wild type isolates and isoniazid-resistant mycobacteria tuberculosis. – Tuberculosis and Lung Diseases. – 2010, N 9: 45-51.
    28. O.Yu. Limanskaya, T.N. Fesenko, V.A. Pokrovskiy, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, A.P. Limanskii. Characterization of oligonucleotides with LNA monomers for PCR detection of point mutations in Mycobacterium tuberculosis genome. – Biochemistry (Moscow). Supplement Series B: Biomedical Chemistry. – 2011. – V. 5, N 2: 144–151.
    29.Limanskaya O., Murtazaeva L., Limanskii A. Species-specific detection of causative agent of anthrax // Biotechnology. – 2012. – V. 5, N 1. – P. 92-99.
    30.O. Limanskaya, A. Limanskii. Study of elongation complexes for T7 RNA polymerase // Biophysics. – 2012. – V. 57, N 4. – P. 428-441.
    31.Limanskaya O., Murtazaeva L., Klee S., Limanskii A. Detection of anthrax causative agent by real-time polymerase chain reaction // Biotechnology. – 2012. – V. 5, N 5. – P. 65-71.
    32.O. Limanskaya, L. Murtazaeva, A. Limanskii. Search for the new potential RNA thermometers in the genome of Salmonella enterica // Microbiology. – 2013. – V. 82, N 1. – P. 69-78.
    33.O.Yu. Limanskaya, L.A. Murtazaeva, and A.P. Limanskii. Potential thermosensitive riboswitches in the genome of Salmonella. - Cytology and Genetics. – 2013. – V. 47, N 5. – P. 268–275.
    PATENTS
    1. Ukraine Patent 36260: A.P. Limanskii, O.Yu. Limanskaya. Detection method of bovine leukemia virus 25.11.1999; Publ.16.04.2001. Bul. N 3.
    2. Ukraine Patent 33324: A.P. Limanskii, O.Yu. Limanskaya, V.F. Babkin, N.D. Bezugly, Yu.L. Vo-lyansky, I.Yu. Kuchma, A.I. Dobroskok, M.V. Babkin. Method of animal embryos sexing 10.02.1999. Publ.15.02.2001. Bul. N 1.
    3. Ukraine Patent 40310: A.P. Limanskii, A.Yu. Volyansky, O.Yu. Limanskaya. Detection method of Listeria monocytogenes, L. Ivanovii, L.seeligeri, L.welshimeri 01.12.2000; Publ.16.07.2001.Bul. N 6.
    4. Ukraine Patent 40371: A.Yu. Volyansky, A.P. Limanskii, O.Yu. Limanskaya. Detection method of pathogenic Listeria sp. 22.12.2000; Publ.16.07.2001. Bul. N 6.
    5. Ukraine Patent 39715: O.Yu. Limanskaya, A.Yu. Volyansky, A.P. Limanskii. Typing method of pathogenic and nonpathogenic Listeria sp. 03.01.2001; Publ.15.06.2001. Bul. N 5.
    6. Ukraine Patent 66255: Tsyganenko A., Limanskaya O., Limanskii A., Mishina M., Minukhin V. Detection method of Proteus vulgaris 09.09.2003; Publ.15.04. 2004. Bul. N 4.
    7. Ukraine Patent 66291: Limanskii A., Limanskaya O., Volyanskii A., Dobroskok A., Novikov S., Manolov V., Volyanskaya N. Detection method of bovine immunodeficiency virus 13.10.2003; Publ.15.04. 2004. Bul. N 4.
    8. Ukraine Patent 13571: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Li-manskaya L., Klimov O. Method of mica (as substrate for atomic force microscopy) modification 22.08.2005; Publ.17.04.2006. Bul. N 4.
    9. Ukraine Patent 17942: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Limanskaya L., Klimov O. Method of stretching linear and supercoiled DNA molecules 25.04.2006; Publ. 16.10.2006. Bul. N 10.
    10. Ukraine Patent 17981: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Kuchma I., Limanskaya L., Pugach N. Method for compression of linear and supercoiled DNA molecules 03.05. 2006; Publ.16.10.2006. Bul. N 10.
    11. Ukraine Patent 80264: Tsyganenko A., Limanskaya O., Limanskii A., Minukhin V. Detection method of Proteus vulgaris and Proteus mirabilis 10.06. 2004; Publ.10.09.2007. Bul. N 14.
    12. Ukraine Patent 34435: Limanskii A., Limanskaya O., Limanskaya L. Differentiation method of mycobacteria tuberculosis complex 17.03. 2008; Publ.11.08.2008. Bul. N 15.
    13. Ukraine Patent 70049: Limanskaya O., Murtazaeva L., Limanskii A. Detection method of anthrax causative agent 14.11.2011; Publ. 25.05.2012. Bul. N 10.
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