National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”

Лаборатория молекулярной диагностики

Laboratory stuff works with complex of problems in sphere of molecular diagnostics, epizootiology and biotechnology. We have three main directions of scientific work in present time. These directions are combined with decision of scientific tasks in the frames of 6 state and 4 international projects.

 

Directions of scientific work:

·  Development and improvement of methodic approaches for infection agents detection for animal viruses and bacteria's in PCR, RFLP, T-step et al., tests validation and diagnostics kits development

 

· Molecular epidemiology study and phylogenetic studies of HPAI viruses, NDV, BVDV, PCV-2, BLV, et al.

 

 

·  Development of biotechnological constructions on the basis of recombinant DNAs

List of state scientific projects (funded by NAAS of Ukraine)

PROJECTS

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”

1. Task 32.01.01.1-01 To study phylogeography and microevolution characteristics of orthomyxo-, paramyxo-, corona-, flavi-, arterio– and circoviral diseases of animals.

 

2. Task 23.3-01.07 To study expression effectively of cassette vectors based on host-specific promoter regions and chimeric genes of animal viruses for recombinant antigens development.

 

3. Task 32.01.02.1-02 To study marker nucleotide sequences of genetic modified plants and to develop system for its screening in the feedstuff, based on PCR.

Chief researcher — A.P. Gerilovych, PhD, SR, ScDr. Projects schedule — 2011-2015.

 

Laboratory of Molecular Diagnostics

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”

Searching for collaboration in HPAI, ND, BVD, PCV-2-infection, viral agents monitoring and agents genotyping, animal genomics and host-specific vector cassettes creation

Laboratory of Molecular

Epizoothology and Diagnostics

List of international projects:

· Comparative molecular study of Polish and Ukrainian BLV isolates (partner—National Veterinary Research Institute, Pulawy, PL, duration 2008-2009, fund. Ministry of Agriculture of Poland)

· FLUTRAIN (partner—Instituto Zooprofilatico Spermientale delle Venezie, Padova, IT, duration 2008-2009, fund. FP-6 EU)

· STCU Grant P168, “Development of Aujeszky disease virus antigen and MAb for ELISA” (partner NPD, USA, duration 2007-2008, fund. STCU)

· STCU Grant P160, " Genetic Variability of Mycobacteria of Tuberculosis and Atypical Mycobacteria Isolated from Farm Animals in Radioactive Polluted Zones” (partner NPD, USA, duration 2007-2008, fund. STCU)

· STCU Grant P 382 “Investigation of Cellular and Molecular Mechanisms of Mucosal Innate Immunity and New Control Strategies Development to Avian-Influenza-Virus”(partner ADPL, USA, duration 2009-2011, fund. STCU)

· STCU Grant P 444 “Monitoring of HPAI, ND, and other paramyxoviruses in Black-sea-Azov region” (partner SEPRL, USA, duration 2010-2012, fund. STCU)

· Swiss National Science Foundation Project SCOPES (NF number IZ730Z0_128050: Epidemiology of BVD in Ukraine: Development of a control program)

· STCU Grant P 382a “Immunostimulation of chicken under plant derived immunostimulators”

· STCU Grant P 453 “Improvement of  infectious animal diseases diagnostics basis (TB and Aujeszky disease)”

· DFFU Grant Study of opisthorchidoses epidemiology